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Handling Effects on Histological Identification of Female Reproductive Status: Examples From Tropical Deepwater Snappers

August 04, 2023

Understanding the differences between handling effects and natural ovarian atresia.

Post-death ovarian tissue degradation from handling effects, such as delays in preservation, can lead to inaccurate reproductive assessments. Therefore, it is important to understand the differences between handling effects and natural ovarian atresia. Seven treatments of various holding temperatures and times to preservation were applied to whole fish and extracted ovaries of three tropical deepwater snapper species.

We advise that (1) freezing ovaries should be avoided where possible, and (2) gonad tissue should ideally be preserved immediately but no later than 48 h post-capture.


Schemmel E, Brown-Peterson NJ 2023. Handling Effects on Histological Identification of Female Reproductive Status: Examples from Tropical Deepwater Snappers Fishes. 2023; 8(8):406. https://doi.org/10.3390/fishes8080406

Last updated by Pacific Islands Fisheries Science Center on 09/05/2023